The Human HGF (Hepatocyte Growth Factor) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of HGF levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.HGF is a key protein involved in promoting cell growth, proliferation, and survival, particularly in hepatocytes. It plays a crucial role in liver regeneration, tissue repair, and development, making it a valuable biomarker for studying liver diseases, cancer, and other conditions. By using the Human HGF CLIA Kit, researchers can gain valuable insights into HGF-mediated pathways, identify potential therapeutic targets, and further understand the role of HGF in various biological processes. This kit offers a convenient and efficient solution for studying HGF levels and its implications in different disease states.
Product Name:
Human HGF (Hepatocyte Growth Factor) CLIA Kit
SKU:
HUES00081
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
