The Human HGF (Hepatocyte Growth Factor) ELISA Kit is specifically designed for the precise quantification of HGF levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for a variety of research applications.Hepatocyte Growth Factor is a critical protein involved in cell growth, tissue regeneration, and wound healing. It plays a key role in liver regeneration and has potential implications in various diseases, including cancer and liver disorders. By measuring HGF levels, researchers can gain insights into disease progression and potential therapeutic interventions.Overall, the Human HGF ELISA Kit from Assay Genie provides researchers with a reliable tool for studying the role of HGF in health and disease, offering valuable information for advancing medical research and developing new treatment strategies.
Product Name:
Human HGF (Hepatocyte Growth Factor) ELISA Kit
SKU:
HUES01370
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
