The Human HPA (Heparanase) ELISA Kit is a specialized assay designed for the precise measurement of heparanase levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Heparanase is a critical enzyme involved in heparan sulfate degradation, which plays a key role in various physiological and pathological processes including tumor metastasis, inflammation, and angiogenesis. By accurately measuring heparanase levels, researchers can gain valuable insights into the role of this enzyme in disease progression and potentially identify new therapeutic targets.Overall, the Human HPA (Heparanase) ELISA Kit is an essential tool for studying the role of heparanase in health and disease, offering reliable and precise measurements to advance scientific research and drug development efforts.
Product Name:
Human HPA (Heparanase) ELISA Kit
SKU:
HUES03236
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
