Human HSD3B7 (3 beta-hydroxysteroid dehydrogenase type 7) ELISA Kit
The Human HSD3B7 (3-Beta-Hydroxysteroid Dehydrogenase Type 7) ELISA Kit is a specialized assay designed for the accurate quantification of HSD3B7 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. HSD3B7 is a key enzyme involved in steroid biosynthesis, specifically in the conversion of pregnenolone to progesterone. Dysregulation of this enzyme has been implicated in a variety of diseases, including certain types of cancer and hormonal disorders.
As such, measuring HSD3B7 levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of novel therapeutic strategies. The Human HSD3B7 ELISA Kit from Assay Genie is a reliable tool for researchers looking to study the role of HSD3B7 in health and disease. With its high sensitivity and accuracy, this kit enables precise quantification of HSD3B7 levels, making it an essential component of any laboratory conducting research in the field of steroid metabolism and related disorders.
Product Name:
Human HSD3B7 (3 beta-hydroxysteroid dehydrogenase type 7) ELISA Kit
SKU:
HUFI03479
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
92-104%
89-104%
92-100%
EDTA Plasma (n = 5)
85-101%
85-98%
82-101%
Heparin Plasma (n = 5)
80-92%
80-94%
80-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-105
97
EDTA Plasma (n = 5)
89-98
93
Heparin Plasma (n = 5)
85-104
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
