Description
Human HSP Array (9 targets) (SARB0046)
The Human HSP Array (9 Targets) is a comprehensive research tool from Assay Genie designed to simultaneously detect multiple Heat Shock Proteins (HSPs) in human samples. This array includes targets such as HSP70, HSP90, and HSP60, which play crucial roles in cellular stress response and protein folding.This high-quality array is optimized for use in protein lysates and cell culture supernatants, allowing for the detection and quantification of HSP expression levels. With its sensitivity and specificity, researchers can easily analyze the dynamics of HSP expression in various disease states, including cancer, neurodegenerative disorders, and cardiovascular diseases.
The Human HSP Array (9 Targets) is a valuable tool for studying the complex network of HSPs and their implications in disease pathology. By providing a comprehensive overview of HSP expression profiles, this array enables researchers to gain insights into the molecular mechanisms underlying cellular stress responses and develop potential therapeutic interventions targeting HSPs.
| Product Sku: | SARB0046 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 9 |
| Gene Symbols: | DNAJB5, GH1, HMOX1, HSP90AA1, HSPA14, HSPA4, HSPB1, HSPE1, RPS27A |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
HSP10
| HSP27
| HSP32
| HSP40
| HSP60
|
HSP70
| HSP90 | GRP75
| Ubiquitin+1
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human HSP Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
