The Kidney Injury Molecule 1 (KIM-1) ELISA Kit is purposefully designed for the precise quantitative assessment of human KIM-1 levels in a variety of biological samples. KIM-1, a transmembrane glycoprotein, serves as a biomarker for kidney injury and is upregulated in response to renal damage. Monitoring KIM-1 levels enables the early detection and evaluation of kidney injury, aiding in the diagnosis and prognosis of renal disorders. Accurate measurement of KIM-1 is crucial for monitoring kidney health, assessing renal function, and guiding appropriate clinical interventions. Our KIM-1 ELISA Kit delivers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research and diagnostic endeavors. Manufactured under stringent quality control standards, this kit guarantees reliable performance and user-friendly protocols, making it an ideal choice for both basic research and clinical applications. Rely on Assay Genie's KIM-1 ELISA Kit for precise and trustworthy quantification of this vital kidney injury biomarker in your studies.
Product Name:
Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit
SKU:
AEES00065
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
