The Mouse Kidney Injury Molecule 1 (KIM-1) ELISA Kit is developed for the precise quantification of KIM-1 levels in various biological samples. KIM-1, a cell surface protein expressed in renal tubular cells, serves as a biomarker for kidney injury and inflammation. It plays a vital role in the kidney's response to injury and can indicate damage to the renal system, making it a valuable indicator for renal health evaluation. This ELISA kit offers exceptional sensitivity and specificity, allowing accurate measurement of KIM-1 levels to assess kidney injury and monitor renal function. With stringent quality control measures, the kit ensures reliable and reproducible results, providing valuable insights into renal pathophysiology. Easy to use and reliable, this kit is an excellent choice for researchers studying kidney diseases and related conditions. (Note: The description includes key information about the kit and its capabilities based on its title and biological context.)
Product Name:
Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit
SKU:
AEES00233
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
