The Rat Kidney Injury Molecule 1 (KIM-1) ELISA Kit is a specialized assay designed for the quantitative measurement of KIM-1 levels in various biological samples obtained from rats. KIM-1, also known as T cell immunoglobulin mucin-1 (TIM-1), is a well-known biomarker for kidney injury and renal damage, playing a crucial role in the early detection of kidney injury and monitoring kidney function. This ELISA kit enables researchers to accurately measure and quantify KIM-1 concentrations, providing valuable insights into kidney injury, renal function, and nephrotoxicity. By offering exceptional sensitivity and specificity, this kit allows for precise and reliable assessment of KIM-1 levels, which is essential for understanding renal pathology, evaluating kidney health, and developing targeted therapeutic strategies for kidney-related disorders. Manufactured under stringent quality control standards, this ELISA kit by Assay Genie delivers robust performance and reproducible results, ensuring accurate and consistent KIM-1 level measurements. With its user-friendly protocols and high-performance characteristics, this kit is an ideal choice for research studies investigating kidney injury, renal biomarkers, and the impact of nephrotoxicity in rat models.
Product Name:
Rat KIM-1 (Kidney Injury Molecule 1) ELISA Kit
SKU:
AEES00533
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
