Human Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit
The Human Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit is specifically designed for the precise quantification of LDLRAP1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring reliable and reproducible results for a variety of research applications.LDLRAP1 is a key adaptor protein involved in the recycling of low-density lipoprotein (LDL) receptors, which play a crucial role in cholesterol metabolism and cardiovascular health.
Dysregulation of LDLRAP1 has been linked to conditions such as familial hypercholesterolemia and other lipid metabolism disorders, making it a valuable biomarker for studying these diseases and potentially developing targeted therapies.With its high-performance qualities and versatility, the Human LDLRAP1 ELISA Kit is an essential tool for researchers studying lipid metabolism, cardiovascular diseases, and other related conditions. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.
Product Name:
Human Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit
Product Code:
HUDL01699
Size:
96 Assays
Target:
LDLRAP1
Synonyms:
ARH1, ARH, ARH2, FHCB1, FHCB2, Autosomal recessive hypercholesterolemia protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
