Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit
The Mouse LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of LDLRAP1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for researchers studying lipid metabolism, cardiovascular diseases, and other related conditions.LDLRAP1 is a key protein involved in the regulation of low-density lipoprotein receptor (LDLR) function, playing a crucial role in cholesterol metabolism and cardiovascular health.
Dysregulation of LDLRAP1 has been linked to conditions such as hypercholesterolemia and atherosclerosis, highlighting its importance as a potential therapeutic target.With its superior performance characteristics and broad range of applications, the Mouse LDLRAP1 ELISA Kit is an essential resource for scientists investigating the molecular mechanisms underlying lipid metabolism and cardiovascular disorders.
Product Name:
Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit
Product Code:
MODL00790
Size:
96 Assays
Target:
LDLRAP1
Synonyms:
ARH1, ARH, ARH2, FHCB1, FHCB2, Autosomal recessive hypercholesterolemia protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
