SynonymsAYTL2,PFAAP3,Acyltransferase Like 2,1-alkylglycerophosphocholine O-acetyltransferase,Phosphonoformate immuno-associated protein 3,Acetyl-CoA:lyso-PAF acetyltransferase
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates And Other Biological Fluids
SKU:
HUDL01744
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8NF37
Sensitivity:
1.15ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
AYTL2,PFAAP3,Acyltransferase Like 2,1-alkylglycerophosphocholine O-acetyltransferase,Phosphonoformate immuno-associated protein 3,Acetyl-CoA:lyso-PAF acetyltransferase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit
The Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit is a powerful tool for accurately measuring levels of LPCAT1 in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring trustworthy and consistent results for a variety of research applications.LPCAT1 is a key enzyme involved in the metabolism of lysophosphatidylcholine (LPC), playing a vital role in lipid biosynthesis and membrane remodeling.
Dysregulation of LPCAT1 has been linked to various diseases including cancer, cardiovascular disorders, and metabolic conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With the Human LPCAT1 ELISA Kit, researchers can accurately measure LPCAT1 levels in human samples, facilitating detailed studies on the role of LPCAT1 in health and disease. Trust this ELISA kit for reliable and reproducible results in your research endeavors.
Product Name:
Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit
Product Code:
HUDL01744
Size:
96 Assays
Target:
LPCAT1
Synonyms:
AYTL2, PFAAP3, Acyltransferase Like 2, 1-alkylglycerophosphocholine O-acetyltransferase, Phosphonoformate immuno-associated protein 3, Acetyl-CoA:lyso-PAF acetyltransferase
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
