SynonymsAYTL2,PFAAP3,Acyltransferase Like 2,1-alkylglycerophosphocholine O-acetyltransferase,Phosphonoformate immuno-associated protein 3,Acetyl-CoA:lyso-PAF acetyltransferase
ReactivityMouse
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
MODL00816
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3TFD2
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AYTL2,PFAAP3,Acyltransferase Like 2,1-alkylglycerophosphocholine O-acetyltransferase,Phosphonoformate immuno-associated protein 3,Acetyl-CoA:lyso-PAF acetyltransferase
The Mouse Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit is specifically designed for the precise measurement of LPCAT1 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.LPCAT1 is an enzyme critical for the biosynthesis of phosphatidylcholine, a major component of cell membranes.
Dysregulation of LPCAT1 has been implicated in diseases such as cancer, metabolic disorders, and inflammation, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.With easy-to-follow protocols and reliable performance, the Mouse LPCAT1 ELISA Kit is an indispensable tool for researchers studying lipid metabolism, membrane biology, and various disease pathways in mouse models.
AYTL2, PFAAP3, Acyltransferase Like 2, 1-alkylglycerophosphocholine O-acetyltransferase, Phosphonoformate immuno-associated protein 3, Acetyl-CoA:lyso-PAF acetyltransferase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
