Human Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit
The Human Lysophosphatidylcholine Acyltransferase-3 (LPCAT3) ELISA Kit is specifically designed for the precise measurement of LPCAT3 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it an excellent tool for various research applications.LPCAT3 is a key enzyme involved in the biosynthesis of phospholipids, playing a crucial role in membrane composition and cell function. Dysregulation of LPCAT3 has been linked to various diseases such as cancer, metabolic disorders, and inflammatory conditions, making it an important biomarker for studying these diseases and potential therapeutic interventions.
This ELISA kit provides researchers with a convenient and efficient method for quantifying LPCAT3 levels, facilitating in-depth analysis of its role in health and disease. With its superior performance and ease of use, the Human LPCAT3 ELISA Kit is a valuable asset for advancing scientific research in lipid metabolism and related fields.
Product Name:
Human Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
