The Human MMP-3 (Matrix Metalloproteinase-3) CLIA Kit is a reliable tool for the quantitative detection of MMP-3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Matrix Metalloproteinase-3, also known as stromelysin-1, is an important enzyme involved in tissue remodeling and degradation of extracellular matrix proteins. Elevated levels of MMP-3 have been associated with various pathological conditions, including cancer, arthritis, and cardiovascular diseases.By accurately measuring MMP-3 levels, researchers can better understand the role of this enzyme in disease progression and potentially identify new therapeutic targets. The Human MMP-3 CLIA Kit is a valuable tool for studying the biology of MMP-3 and developing novel treatment strategies for related disorders.
Product Name:
Human MMP-3 (Matrix Metalloproteinase 3) CLIA Kit
SKU:
HUES00823
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
