The Human MMP-8 (Matrix Metalloproteinase-8) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of MMP-8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.MMP-8, also known as collagenase-2, is a key enzyme involved in the breakdown of extracellular matrix proteins and tissue remodeling. Elevated levels of MMP-8 have been associated with various inflammatory and pathological conditions, including arthritis, periodontal disease, and cancer.
This makes MMP-8 a valuable biomarker for studying these diseases and developing potential therapeutic interventions.With its advanced technology and reliable performance, the Human MMP-8 CLIA Kit is an indispensable tool for researchers and clinicians seeking to investigate the role of MMP-8 in disease pathology and progression. Order yours today to unlock new insights into the complex mechanisms of MMP-8 and its impact on human health.
Product Name:
Human MMP-8 (Matrix Metalloproteinase 8) CLIA Kit
SKU:
HUES00827
Target:
Human MMP-8 (Matrix Metalloproteinase 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-8. You can calculate the concentration of Human MMP-8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
95-108
94-110
Average (%)
101
102
100
1:4
Range (%)
98-116
94-107
84-96
Average (%)
106
101
90
1:8
Range (%)
94-104
99-113
99-115
Average (%)
99
105
107
1:16
Range (%)
89-101
86-97
95-113
Average (%)
94
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
92-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.45
171.13
670.93
102.85
179.85
713.09
Standard deviation
8.59
12.49
53.41
10.23
13.06
64.96
C V (%)
8.73
7.3
7.96
9.95
7.26
9.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-8 in samples. No significant cross-reactivity or interference between Human MMP-8 and analogues was observed.
