Human MMP-8 (Matrix Metalloproteinase 8) ELISA Kit
The Human MMP-8 (Matrix Metalloproteinase 8) ELISA Kit is a reliable tool for the quantitative measurement of MMP-8 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for your research needs.MMP-8 is a key enzyme that plays a crucial role in the breakdown of extracellular matrix proteins, leading to tissue remodeling and turnover. Its dysregulation has been implicated in various pathological conditions, including inflammation, wound healing, and cancer progression. Therefore, the detection of MMP-8 levels can provide valuable insights into these disease processes and facilitate the development of targeted therapies.By using the Human MMP-8 ELISA Kit, researchers can gain a better understanding of MMP-8 expression levels in different samples and investigate its potential as a biomarker for disease diagnosis and prognosis. This kit is a valuable addition to any laboratory conducting research in the fields of cancer biology, inflammation, and tissue regeneration.
Product Name:
Human MMP-8 (Matrix Metalloproteinase 8) ELISA Kit
SKU:
HUES02485
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
