Human MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
The Human MMP-1 (Matrix Metalloproteinase 1) ELISA Kit is expertly crafted for the quantitative detection and measurement of Matrix Metalloproteinase-1 levels in various biological samples. MMP-1, an essential member of the matrix metalloproteinase family, plays a pivotal role in extracellular matrix remodeling and degradation, associated with tissue repair, cellular migration, and various physiological and pathological processes. Accurate quantification of MMP-1 with this ELISA kit provides valuable insights into tissue remodeling, wound healing, and pathological conditions such as cancer metastasis, arthritis, and cardiovascular diseases. The kit ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. With stringent quality control measures, this kit offers robust performance and ease of use, making it an ideal choice for both research and clinical applications. Trust the Human MMP-1 ELISA Kit for precise and dependable measurement of this critical biomarker in your scientific investigations.
Product Name:
Human MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
SKU:
AEES00109
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.