Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
The Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit is expertly designed for the precise and quantitative assessment of Matrix Metalloproteinase 9 levels in a variety of biological samples. Matrix Metalloproteinase 9, a crucial enzyme, is known for its role in extracellular matrix remodeling, tissue repair, and a spectrum of physiological and pathological processes. This ELISA kit enables accurate detection of MMP-9, providing valuable insights into processes such as tissue remodeling, cell migration, and inflammation. With exceptional sensitivity and specificity, the kit ensures reliable and reproducible results. Manufactured under strict quality control measures, this kit offers robust performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Trust the Human MMP-9 ELISA Kit for accurate and dependable quantification of this vital enzyme in your studies.
Product Name:
Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
SKU:
AEES00111
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
