The Rat Matrix Metalloproteinase 9 (MMP-9) ELISA Kit is a specialized assay designed to accurately quantify MMP-9 levels in various biological samples derived from rat models. MMP-9, also known as Gelatinase B, is a critical member of the matrix metalloproteinase family that plays a crucial role in the degradation and remodeling of extracellular matrix components. This ELISA kit enables researchers to explore the activity and concentration of MMP-9, providing valuable insights into tissue remodeling, wound healing, angiogenesis, and inflammatory responses. The accurate measurement of MMP-9 levels using this kit is essential for understanding its involvement in various physiological and pathological processes, including cancer metastasis, tissue repair, and inflammatory disorders. Manufactured under strict quality control standards, this ELISA kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Researchers can rely on this kit to deliver robust performance, precise measurements, and ease of use, making it an excellent choice for investigating the role of MMP-9 in rat models and understanding its implications in different biological contexts.
Product Name:
Rat MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
SKU:
AEES00535
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
