Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
The Matrix Metalloproteinase 13 (MMP-13) ELISA Kit is designed to accurately quantify MMP-13 levels in human samples. MMP-13, a collagenase enzyme that plays a crucial role in extracellular matrix remodeling and tissue maintenance, is associated with various biological processes, including tissue repair, inflammation, and cancer metastasis. Elevated levels of MMP-13 have been linked to pathological conditions such as arthritis, cancer invasion, and wound healing abnormalities. With exceptional sensitivity and specificity, the MMP-13 ELISA Kit provides reliable and reproducible results, enabling precise measurement of MMP-13 concentrations in biological samples. Manufactured under strict quality controls, this kit ensures robust performance and ease of use, making it ideal for both research and clinical applications. Trust Assay Genie's MMP-13 ELISA Kit for accurate and consistent quantification of this important biomarker in your studies.
Product Name:
Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
SKU:
AEES00059
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
