The Mouse MMP-13 (Matrix Metalloproteinase-13) ELISA Kit is designed for the precise quantification of MMP-13 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MMP-13 is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a critical role in tissue remodeling and repair processes. Dysregulation of MMP-13 has been linked to various pathological conditions, including arthritis, cancer, and inflammatory diseases, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.With its advanced technology and ease of use, the Mouse MMP-13 ELISA Kit from AssayGenie is an essential tool for researchers studying MMP-13 biology and its implications in disease pathogenesis.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
