Rat MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
The Rat Matrix Metalloproteinase 13 (MMP-13) ELISA Kit is innovatively designed to quantitatively detect MMP-13 levels in a variety of rat biological samples. MMP-13, a prominent member of the matrix metalloproteinase family, plays a crucial role in extracellular matrix remodeling by cleaving various matrix components. With its involvement in tissue homeostasis, repair, and inflammatory processes, MMP-13 serves as a key regulator in maintaining tissue integrity and function. This ELISA kit provides researchers with a reliable and accurate tool for assessing MMP-13 levels, offering valuable insights into its contribution to physiological and pathological conditions, including arthritis, cancer metastasis, and tissue regeneration. Boasting exceptional sensitivity and specificity, the assay ensures robust and reproducible results, facilitating in-depth investigations into MMP-13 biology and its implications in various disease mechanisms. Manufactured under strict quality controls, this kit promises reliable performance, making it a preferred choice for research studies focused on matrix dynamics and tissue remodeling.
Product Name:
Rat MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
SKU:
AEES00306
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
