The Rat MMP-3 (Matrix Metalloproteinase 3) ELISA Kit is a powerful tool designed for the precise and quantitative measurement of Matrix Metalloproteinase 3 in various rat biological samples. Matrix Metalloproteinase 3, also known as Stromelysin-1, is a key enzyme involved in extracellular matrix degradation, tissue remodeling, and cell migration processes. Its dysregulation has been linked to various inflammatory and pathological conditions. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs. Developed with stringent quality control standards, the Rat MMP-3 ELISA Kit by Assay Genie delivers reliable performance and user-friendly protocols, making it an excellent choice for investigating the role of Matrix Metalloproteinase 3 in disease progression, tissue repair, and other physiological processes. Trust Assay Genie's expertise to advance your research in Matrix Metalloproteinase 3 biology.
Product Name:
Rat MMP-3 (Matrix Metalloproteinase 3) ELISA Kit
SKU:
AEES00480
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
