The Mouse MMP-3 (Matrix Metalloproteinase-3) ELISA Kit is a specialized assay designed for the quantitative measurement of MMP-3 levels in mouse biological samples including serum, plasma, and tissue homogenates. This kit offers exceptional accuracy and precision, delivering reliable and consistent results for a variety of experimental needs.Matrix Metalloproteinase-3, also known as stromelysin-1, is a key enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a critical role in processes such as wound healing, inflammation, and cancer progression. By accurately measuring MMP-3 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and specificity, the Mouse MMP-3 ELISA Kit is a valuable tool for studying the role of MMP-3 in various physiological and pathological conditions. Whether investigating arthritis, cardiovascular diseases, or cancer, this kit provides a reliable means of quantifying MMP-3 levels and advancing research in the field of matrix metalloproteinases.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
