Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit
The Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit is designed for the precise measurement of MMP-3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results, making it an indispensable tool for a variety of research applications.MMP-3, also known as stromelysin-1, is a key enzyme involved in tissue remodeling and extracellular matrix degradation. Its dysregulation has been linked to various pathological conditions including arthritis, cardiovascular diseases, and cancer. By measuring MMP-3 levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.Overall, the Human MMP-3 ELISA Kit provides researchers with a reliable and efficient way to study the role of MMP-3 in various diseases, ultimately advancing our understanding of disease mechanisms and aiding in the development of novel treatment strategies.
Product Name:
Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit
SKU:
HUES02481
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
