The Rat Matrix Metalloproteinase 8 (MMP-8) ELISA Kit is a specialized assay designed for the quantitative measurement of MMP-8 levels in various biological samples derived from rat models. MMP-8, also known as Collagenase-2, is a crucial member of the matrix metalloproteinase family involved in the degradation and remodeling of extracellular matrix components, particularly type I collagen. This ELISA kit enables researchers to accurately quantify MMP-8 concentrations, providing valuable insights into tissue remodeling, inflammatory responses, wound healing, and extracellular matrix metabolism. By offering exceptional sensitivity and specificity, this kit facilitates reliable and reproducible measurement of MMP-8 levels, which is vital for understanding its role in physiological and pathological processes. Manufactured under stringent quality control standards, this ELISA kit by Assay Genie delivers robust and consistent performance, ensuring precise and accurate measurement of MMP-8 levels in rat biological samples. With user-friendly protocols and high-performance features, this kit is an excellent tool for investigating the involvement of MMP-8 in various disease conditions, tissue remodeling processes, and inflammatory responses in rat models.
Product Name:
Rat MMP-8 (Matrix Metalloproteinase 8) ELISA Kit
SKU:
AEES00534
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
