The Rat MMP-2 (Matrix Metalloproteinase 2) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Matrix Metalloproteinase 2 in various rat biological samples. Matrix Metalloproteinase 2 is a crucial enzyme that plays a significant role in tissue remodeling, extracellular matrix degradation, and cell migration processes. As a key member of the matrix metalloproteinase family, MMP-2 is involved in various physiological and pathological conditions. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research. Developed under stringent quality control measures, the Rat MMP-2 ELISA Kit by Assay Genie provides robust performance and ease of use, making it an ideal choice for investigating the role of Matrix Metalloproteinase 2 in disease mechanisms, wound healing, and tissue repair processes. Trust Assay Genie's expertise to advance your research in Matrix Metalloproteinase 2 biology.
Product Name:
Rat MMP-2 (Matrix Metalloproteinase 2) ELISA Kit
SKU:
AEES00479
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
