The Mouse MMP-2 (Matrix Metalloproteinase 2) ELISA Kit is specifically designed for the accurate detection of MMP-2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MMP-2 is a critical enzyme involved in extracellular matrix remodeling, cell migration, and tissue repair. Dysregulation of MMP-2 has been linked to various pathologies, including cancer, cardiovascular diseases, and inflammatory disorders, making it a valuable biomarker for understanding disease mechanisms and developing novel therapeutic strategies. With easy-to-follow protocols and rapid assay times, the Mouse MMP-2 ELISA Kit from Assay Genie is a reliable tool for investigating MMP-2 levels in experimental models and clinical samples. Order now to accelerate your research and advance our understanding of MMP-2 biology.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
