The Rat MMP-1 (Matrix Metalloproteinase 1) ELISA Kit is a powerful tool designed for the precise and quantitative measurement of Matrix Metalloproteinase 1 levels in various rat biological samples. Matrix Metalloproteinase 1 is a key enzyme responsible for degrading components of the extracellular matrix, playing essential roles in tissue remodeling, wound healing, and various physiological processes. This ELISA kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research experiments. Manufactured under strict quality control to deliver reliable and robust performance, the Assay Genie's MMP-1 ELISA Kit offers researchers a user-friendly and effective solution to investigate the functions and regulatory mechanisms of Matrix Metalloproteinase 1 in biological pathways and disease conditions. Trust in Assay Genie for high-quality ELISA kits that drive cutting-edge research in Matrix Metalloproteinase biology.
Product Name:
Rat MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
SKU:
AEES00478
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
