Human mTOR (Mammalian Target of Rapamycin) ELISA Kit
The Human mTOR (Mammalian Target of Rapamycin) ELISA Kit is specifically designed for the accurate detection of mTOR levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.mTOR is a key protein involved in regulating cell growth, metabolism, and survival, making it a critical target for studying diseases such as cancer, diabetes, and neurological disorders. By accurately measuring mTOR levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human mTOR ELISA Kit from Assay Genie provides researchers with a reliable tool to investigate the role of mTOR in various physiological and pathological processes, paving the way for novel discoveries and advancements in biomedical research.
Product Name:
Human mTOR (Mammalian Target of Rapamycin) ELISA Kit
SKU:
HUES02655
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
