Human mTOR (Mammalian Target of Rapamycin) CLIA Kit
The Human mTOR (Mammalian Target of Rapamycin) CLIA Kit is specifically designed for the accurate detection of mTOR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.mTOR is a vital protein that regulates cell growth, proliferation, and metabolism, playing a crucial role in various cellular processes. Dysregulation of mTOR has been linked to numerous diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human mTOR CLIA Kit from AssayGenie provides researchers with a powerful tool to investigate the role of mTOR in health and disease, facilitating advancements in scientific research and potential therapeutic strategies.
Product Name:
Human mTOR (Mammalian Target of Rapamycin) CLIA Kit
SKU:
HUES00931
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
