Description
Human Obesity Array (62 targets) (SARB0015)
The Human Obesity Array (62 targets) is a powerful tool for researchers studying obesity and related metabolic disorders. This array allows for the simultaneous detection and quantification of 62 different proteins involved in processes such as adipogenesis, glucose metabolism, inflammation, and hormone regulation.With targets including adiponectin, leptin, insulin, and TNF-alpha, this array provides a comprehensive overview of the molecular mechanisms underlying obesity. By analyzing the expression levels of these key proteins, researchers can gain insights into the pathophysiology of obesity and identify potential therapeutic targets.
The Human Obesity Array is suitable for use with a variety of sample types, including serum, plasma, and tissue lysates. Its high sensitivity and specificity ensure reliable results, making it an essential tool for studies aiming to unravel the complexities of obesity and develop new treatments for this global health epidemic.
| Product Sku: | SARB0015 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 62 |
| Gene Symbols: | ACE2, ADIPOQ, AGRP, ANGPT1, ANGPT2, ANGPTL4, CCL16, CCL18, CCL2, CCL25|CCL4, CCL5, CCL7, CFD, CRP, CSF1, CXCL10, CXCL12, CXCL5, CXCL8, EDA2R, FAS, FGF6, GH1, IFNG, IGF1, IGF1R, IGFBP1, IGFBP2, IGFBP3, IL10, IL11, IL12A, IL1A, IL1B, IL1R1, IL1RL1, IL6, IL6R, INS, LEP, LEPR, LIF, MIF, MST1, OSM, PDGFA, PDGFB, RETN, SAA1, SERPINE1, TGFB1, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF9, TNFSF9, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Targets Included | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137) | ACE-2 | Adiponectin (ACRP30) | Adipsin (Complement Factor D) | AgRP |
| Angiopoietin-1 | Angiopoietin-2 | ANGPTL4 | CRP (C-Reactive Protein) | ENA-78 (CXCL5) |
| Fas (TNFRSF6/Apo-1) | FGF-6 | Growth Hormone | HCC-4 (CCL16) | IFN-gamma |
| IGFBP-1 | IGFBP-2 | IGFBP-3 | IGF-1 | IGF-1 R |
| IL-1 R4 (ST2) | IL-1 R1 | IL-10 | IL-11 | IL-12 p70 |
| IL-1 alpha (IL-1 F1) | IL-1 beta (IL-1 F2) | IL-6 | IL-6 R | IL-8 (CXCL8) |
| Insulin | IP-10 (CXCL10) | Leptin R | Leptin | LIF |
| Lymphotactin (XCL1) | MCP-1 (CCL2) | MCP-3 (MARC/CCL7) | M-CSF | MIF |
| MIP-1 beta (CCL4) | MSP alpha/beta | Osteoprotegerin (TNFRSF11B) | Oncostatin M | PAI-1 |
| PARC (CCL18) | PDGF-AA | PDGF-AB | PDGF-BB | RANTES (CCL5) |
| Resistin | SAA (Serum Amyloid A) | SDF-1 alpha (CXCL12 alpha) | TNF RII (TNFRSF1B) | TNF RI (TNFRSF1A) |
| TECK (CCL25) | TGF beta 1 | TIMP-1 | TIMP-2 | TNF alpha |
| VEGF-A | XEDAR | |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Obesity Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
