Human PDGF-BB (Platelet Derived Growth Factor-BB) CLIA Kit
The Human PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit is a highly sensitive and specific assay designed to accurately quantify PDGF-BB levels in human samples such as serum, plasma, and cell culture supernatants. This kit is essential for research applications related to cell growth, differentiation, and tissue repair.PDGF-BB is a key growth factor that plays a vital role in various cellular processes, including wound healing, tissue regeneration, and the development of blood vessels.
Its dysregulation has been implicated in diseases such as cancer, fibrosis, and vascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its reliable performance and user-friendly format, the Human PDGF-BB CLIA Kit from AssayGenie facilitates accurate and reproducible measurements of PDGF-BB levels, enabling researchers to gain valuable insights into the role of this growth factor in health and disease.
Product Name:
Human PDGF-BB (Platelet Derived Growth Factor-BB) CLIA Kit
SKU:
HUES00894
Target:
Human PDGF-BB (Platelet Derived Growth Factor-BB)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-BB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDGF-BB. You can calculate the concentration of Human PDGF-BB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
89-100
97-108
Average (%)
102
94
102
1:4
Range (%)
94-108
101-116
87-99
Average (%)
100
107
93
1:8
Range (%)
93-107
95-106
100-114
Average (%)
98
101
106
1:16
Range (%)
87-103
97-109
86-99
Average (%)
94
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
97-114
105
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.18
155.06
370.18
50.53
159.97
399.15
Standard deviation
4.67
15.6
31.5
5.29
17.24
27.02
C V (%)
8.95
10.06
8.51
10.47
10.78
6.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDGF-BB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDGF-BB in samples. No significant cross-reactivity or interference between Human PDGF-BB and analogues was observed.
