Rat PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit
The Rat PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is a specialized assay designed for the quantitative measurement of Platelet Derived Growth Factor BB levels in various biological samples. PDGF-BB, a potent growth factor, is involved in crucial cellular activities such as cell proliferation, migration, and angiogenesis. It plays a significant role in tissue repair, wound healing, and normal development processes. Accurate detection and quantification of PDGF-BB are essential for understanding its biological functions and its implications in physiological and pathological conditions. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit provides reliable performance and user-friendly protocols, making it an ideal choice for cutting-edge research applications.
Product Name:
Rat PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit
SKU:
AEES00446
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
