Rat PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit
The Rat PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit is a state-of-the-art assay designed for the accurate quantification of PDGF-BB levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a wide range of research applications.PDGF-BB is a critical growth factor that plays a key role in various biological processes, including cell growth, proliferation, and wound healing. Dysregulation of PDGF-BB is associated with conditions such as cancer, fibrosis, and vascular diseases, making it a valuable biomarker for studying disease pathogenesis and developing targeted therapies.With its advanced technology and ease of use, the Rat PDGF-BB CLIA Kit from AssayGenie is an essential tool for researchers investigating the role of PDGF-BB in health and disease. Trust in AssayGenie for high-quality and reliable research tools to advance your scientific discoveries.
Product Name:
Rat PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit
SKU:
RTES00322
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
