The Mouse PDGF-BB (Platelet-Derived Growth Factor-BB) ELISA Kit is a reliable and accurate assay designed for the quantitative detection of PDGF-BB levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PDGF-BB is a key growth factor involved in cell proliferation, migration, and angiogenesis, playing a crucial role in tissue repair and regeneration. Dysregulation of PDGF-BB signaling has been implicated in various diseases, including cancer, cardiovascular disorders, and fibrotic conditions, making it a valuable biomarker for studying these pathologies and developing targeted therapies. The Mouse PDGF-BB ELISA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of PDGF-BB in physiological and pathological processes, and for evaluating potential therapeutic interventions. Trust in the accuracy and reliability of this kit to deliver meaningful data for advancing your research.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
