Human PDGF-BB (Platelet Derived Growth Factor-BB) ELISA Kit
The Human PDGF-BB (Platelet-Derived Growth Factor BB) ELISA Kit is specifically designed for the precise quantification of PDGF-BB levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research applications.PDGF-BB is a key growth factor that plays a critical role in regulating cell growth, proliferation, and differentiation. It is essential for wound healing, tissue repair, and maintaining normal cell function. Dysregulation of PDGF-BB expression has been implicated in various diseases such as cancer, fibrosis, and cardiovascular disorders, making it a valuable biomarker for investigating disease mechanisms and developing potential therapeutic interventions. By utilizing the Human PDGF-BB ELISA Kit, researchers can confidently measure PDGF-BB levels in biological samples with precision, facilitating in-depth studies on the role of PDGF-BB in health and disease. This kit is a valuable tool for advancing scientific understanding and driving progress in biomedical research.
Product Name:
Human PDGF-BB (Platelet Derived Growth Factor-BB) ELISA Kit
SKU:
HUES02588
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
