Description
Human Perforin/PRF1 Recombinant Protein (N-His)
Human Perforin/PRF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln411-Gly531, with an N-terminal His tag. Predicted molecular weight: 15.95 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Perforin-1 (PRF1) is a ~61 kDa protein. Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. For research use only.
Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes.
| Product Name: | Human Perforin/PRF1 Recombinant Protein (N-His) |
| SKU: | GXRP00971 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Cytolysin, Perforin-1, Lymphocyte pore-forming protein, PFP, PRF1, P1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln411-Gly531 |
| Calculated MW: | 15.95 kDa |
| UniProt: | P14222 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
