Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
The Assay Genie Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit has been meticulously crafted for the precise quantification of RANTES levels in diverse biological samples. RANTES, known as Regulated On Activation, Normal T-Cell Expressed and Secreted, is a pivotal chemokine involved in immune regulation, inflammation, and the recruitment of immune cells to sites of inflammation or infection. By accurately measuring RANTES levels, researchers can gain valuable insights into the immune response, inflammatory processes, and various immune-mediated disorders, enabling the development of targeted therapeutic interventions in these essential areas. Our RANTES ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your scientific investigations. Manufactured under stringent quality control measures, this kit delivers reliable performance and user-friendly procedures, making it a preferred choice for conducting impactful research in immunology and related fields. Trust Assay Genie's RANTES ELISA Kit for dependable and precise quantification, empowering your exploration into the roles of this critical chemokine.
Product Name:
Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
SKU:
AEES00179
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
