Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
The Mouse Rantes (Regulated on Activation, Normal T Cell Expressed and Secreted) ELISA Kit is specifically designed for the precise measurement of Rantes levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research applications.Rantes is a key chemokine involved in immune responses and inflammation, playing a critical role in recruiting leukocytes to sites of injury or infection. Dysregulation of Rantes has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and viral infections. The Mouse Rantes ELISA Kit provides researchers with a valuable tool for investigating the role of Rantes in these diseases and potential therapeutic interventions.
Product Name:
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
SKU:
MOES00630
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
