SynonymsCHC1L,RLG,RCC1-like G exchanging factor,Regulator Of Chromosome Condensation And BTB(POZ)Domain Containing Protein 2,Chromosome Condensation 1-Like
ReactivityHuman
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
HUDL02515
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O95199
Sensitivity:
0.124ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CHC1L,RLG,RCC1-like G exchanging factor,Regulator Of Chromosome Condensation And BTB(POZ)Domain Containing Protein 2,Chromosome Condensation 1-Like
Human RCC1 And BTB Domain Containing Protein 2 (RCBTB2) ELISA Kit
The Human RCC1 and BTB Domain-Containing Protein 2 (RCBTB2) ELISA Kit is specifically designed for the precise measurement of RCBTB2 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an essential tool for various research applications. RCBTB2 is a critical protein involved in various cellular processes, including cell cycle regulation and protein degradation.
Dysregulation of RCBTB2 has been linked to cancer development and progression, making it a valuable biomarker for studying cancer biology and potential therapeutic interventions. Harness the power of the Human RCC1 and BTB Domain-Containing Protein 2 (RCBTB2) ELISA Kit to advance your research in cancer biology and beyond.
Product Name:
Human RCC1 And BTB Domain Containing Protein 2 (RCBTB2) ELISA Kit
Product Code:
HUDL02515
Size:
96 Assays
Target:
RCBTB2
Synonyms:
CHC1L, RLG, RCC1-like G exchanging factor, Regulator Of Chromosome Condensation And BTB(POZ)Domain Containing Protein 2, Chromosome Condensation 1-Like
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
