Description
Human RIPK2 Recombinant Protein (N-GST)
Human RIPK2 Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Gln432-Met540, with an N-terminal GST tag. Predicted molecular weight: 39.28 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) is a ~61 kDa protein. Serine/threonine/tyrosine-protein kinase that plays an essential role in modulation of innate and adaptive immune responses. For research use only.
Acts as a key effector of NOD1 and NOD2 signaling pathways: upon activation by bacterial peptidoglycans, NOD1 and NOD2 oligomerize and recruit RIPK2 via CARD-CARD domains, leading to the formation of RIPK2 filaments. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3, as well as 'Met-1'-linked (linear) polyubiquitination by the LUBAC complex, becoming a scaffolding protein for downstream effectors. 'Met-1'-linked polyubiquitin chains attached to RIPK2 recruit IKBKG/NEMO, which undergoes 'Lys-63'-linked polyubiquitination in a RIPK2-dependent process. 'Lys-63'-linked polyubiquitin chains attached to RIPK2 serve as docking sites for TAB2 and TAB3 and mediate the recruitment of MAP3K7/TAK1 to IKBKG/NEMO, inducing subsequent activation of IKBKB/IKKB.
| Product Name: | Human RIPK2 Recombinant Protein (N-GST) |
| SKU: | GXRP03352 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Tyrosine-protein kinase RIPK2, CARDIAK, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, RICK, RIPK2, CARD-containing IL-1 beta ICE-kinase, Receptor-interacting serine/threonine-protein kinase 2, RIP-like-interacting CLARP kinase, RIP2, Receptor-interacting protein 2, RIP-2 |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Gln432-Met540 |
| Calculated MW: | 39.28 kDa |
| UniProt: | O43353 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
