Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit
The Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit is a reliable and efficient tool for the precise measurement of S100A12 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.S100A12 is a key protein involved in inflammatory processes, immune response regulation, and cell differentiation. Its levels have been implicated in various diseases, including inflammatory disorders, autoimmune diseases, and cardiovascular conditions. Monitoring S100A12 levels can provide valuable insights into disease progression and treatment efficacy, making it a valuable biomarker for research and clinical applications.
Product Name:
Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit
SKU:
HUES00745
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
