Porcine S100 Calcium Binding Protein A12 (S100A12) ELISA Kit
The Porcine S100 Calcium Binding Protein A12 (S100A12) ELISA Kit is a powerful tool for the accurate measurement of S100A12 levels in porcine samples such as serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a wide range of research applications.S100A12 is a calcium-binding protein that plays a critical role in inflammatory processes and immune regulation. Elevated levels of S100A12 have been linked to various inflammatory conditions such as arthritis, inflammatory bowel disease, and sepsis, making it a valuable biomarker for studying these disorders and developing targeted therapies.
With this advanced ELISA kit, researchers can confidently assess S100A12 levels in porcine samples, gaining valuable insights into the role of this important protein in inflammation and disease pathogenesis. Trust in the Porcine S100A12 ELISA Kit for accurate and precise measurement of S100A12 levels in your research studies.
Product Name:
Porcine S100 Calcium Binding Protein A12 (S100A12) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
