Human S100B (S100 Calcium Binding Protein B) CLIA Kit
The Human S100B (S100 Calcium Binding Protein B) CLIA Kit is specifically designed for the precise measurement of S100B levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.S100B is a key protein that belongs to the S100 family and plays a critical role in calcium signaling and regulating cell functions.
It is implicated in various neurological disorders, including traumatic brain injury, Alzheimer's disease, and schizophrenia, making it a valuable biomarker for investigating these conditions and identifying potential treatment approaches.Overall, the Human S100B CLIA Kit provides researchers with a powerful tool to study the role of S100B in health and disease, offering reliable data for further exploration and therapeutic development.
Product Name:
Human S100B (S100 Calcium Binding Protein B) CLIA Kit
SKU:
HUES00749
Target:
Human S100B (S100 Calcium Binding Protein B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
95-108
89-102
Average (%)
102
102
94
1:4
Range (%)
97-113
93-107
100-114
Average (%)
105
99
107
1:8
Range (%)
85-97
85-98
93-104
Average (%)
92
90
98
1:16
Range (%)
85-99
96-111
87-99
Average (%)
92
102
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
91-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.86
97.33
353.43
48.09
100.17
365.56
Standard deviation
4.45
10.45
31.7
3.97
8.45
22.19
C V (%)
8.58
10.74
8.97
8.26
8.44
6.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100B in samples. No significant cross-reactivity or interference between Human S100B and analogues was observed.
