Human S100B (S100 Calcium Binding Protein B) ELISA Kit
The Human S100B (S100 Calcium Binding Protein B) ELISA Kit is a highly reliable tool for the precise measurement of S100B levels in human samples, including serum, plasma, and cell culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.S100B is a vital protein that acts as a calcium-binding protein in the body, playing a crucial role in various physiological processes. It is known to be involved in conditions such as brain injuries, neurodegenerative diseases, and psychiatric disorders, making it an indispensable biomarker for investigating these conditions and potential therapeutic interventions.Overall, the Human S100B ELISA Kit offers researchers a valuable tool for studying the role of S100B in human health and disease, providing reliable data for further understanding its impact on various pathological processes.
Product Name:
Human S100B (S100 Calcium Binding Protein B) ELISA Kit
SKU:
HUES02353
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
