Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit
The Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit is a reliable and precise tool for quantifying levels of S100B in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable resource for various research endeavors.S100B is a calcium-binding protein that plays a crucial role in various cellular processes, including neuronal function and inflammation. Its dysregulation has been implicated in a range of neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. This ELISA kit enables researchers to explore the role of S100B in disease progression and treatment response, providing valuable insights into its potential as a therapeutic target. With its user-friendly protocol and precise measurements, the Mouse S100B ELISA Kit is a valuable addition to any laboratory conducting research in the field of neuroscience and neurodegenerative diseases.
Product Name:
Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit
SKU:
MOES01455
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.