Rat S100B (S100 Calcium Binding Protein B) ELISA Kit
The Rat S100B (S100 Calcium Binding Protein B) ELISA Kit is specifically designed for the accurate quantification of S100B levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing reliable and reproducible results for various research applications.S100B is a key protein involved in regulating cell growth, differentiation, and inflammation. Elevated levels of S100B have been implicated in various neurological disorders, making it a valuable biomarker for studying conditions such as brain injury, neurodegenerative diseases, and psychiatric disorders. By using the Rat S100B ELISA Kit, researchers can gain valuable insights into the role of S100B in these conditions and potentially identify new therapeutic targets for treatment. This kit is easy to use and provides accurate measurements, making it an essential tool for scientists working in the field of neuroscience and neurology.
Product Name:
Rat S100B (S100 Calcium Binding Protein B) ELISA Kit
SKU:
RTES00723
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
