The Human SEMA3B (Semaphorin 3B) ELISA Kit is specifically designed for the precise quantification of SEMA3B levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for a diverse range of research applications. SEMA3B is a critical protein involved in cell signaling and tissue development, playing a key role in regulating cell migration and axon guidance. Dysregulation of SEMA3B expression has been linked to various diseases, including cancer, neurological disorders, and cardiovascular conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
By utilizing the Human SEMA3B ELISA Kit, researchers can gain valuable insights into SEMA3B levels, furthering our understanding of its role in disease progression and facilitating the development of targeted treatments. Trust in the accuracy and reliability of this ELISA kit to advance your research endeavors in the field of SEMA3B biology.
Product Name:
Human Semaphorin 3B/SEMA3B ELISA Kit
SKU:
HUFI01855
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
90-105%
83-98%
82-94%
EDTA Plasma (n = 5)
85-101%
83-98%
80-93%
Heparin Plasma (n = 5)
87-103%
84-98%
87-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-100
91
EDTA Plasma (n = 5)
92-104
99
Heparin Plasma (n = 5)
93-102
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
