Human Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit
The Human Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit is a powerful tool for the precise measurement of SGPP2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it perfect for a wide array of research applications.SGPP2 is a key enzyme involved in sphingolipid metabolism, specifically in the regulation of the bioactive lipid sphingosine-1-phosphate (S1P). Dysregulation of SGPP2 has been implicated in various diseases including cancer, autoimmune disorders, and neurodegenerative conditions.
By accurately measuring SGPP2 levels, researchers can gain valuable insights into these diseases and potentially discover new therapeutic targets.Overall, the Human SGPP2 ELISA Kit is an indispensable tool for researchers looking to study sphingolipid metabolism and its role in disease pathology. Its accuracy and reliability make it a valuable asset in the quest for better understanding and treatment of various health conditions.
Product Name:
Human Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit
Product Code:
HUDL02667
Size:
96 Assays
Target:
SGPP2
Synonyms:
SPP2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
